Short communication: Investigation of mutation in intron 1 of myostatin gene in Lori sheep using PCR-SSCP method

Authors
F. Sepahvand
M.T. Beiginassiri
J. Fayazi
M. Khaldari
10.22077/jlr.2014.259
Abstract
The objective of this study was to detect mutation in intron 1 of myostatin gene using 110 individuals of Lori sheep breed. A 414-bp fragment of myostatin gene intron 1 was amplified and evaluated using PCR-SSCP. Five different SSCP patterns (genotype) and five alleles were observed. Frequencies of alleles A, D, C, E and F were 0.69, 0.19, 0.07, 0.02 and 0.03, respectively. Frequencies of genotype AA, AD, AC, AE and AF were 0.38, 0.36, 0.15, 0.05 and 0.06, respectively. Heterozygosity value was found to be 0.61. The chi-square test showed that the population has been deviated from Hardy- Weinberg equilibrium for the locus studied (P<0.05).
Keywords
Myostatin gene
Polymerase chain reaction
Single strand conformation polymorphism
Lori sheep
Polymorphism